报告题目：Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N
报告人：Dr. Jingjun Hong
Research Fellow, National Cancer Institute, NIH, Bethesda, MD
报告摘要：Accurate chromosome segregation requires the proper assembly of kinetochore proteins. A key step in this process is the recognition of the histone H3 variant CENP-A in the centromeric nucleosome by the kinetochore protein CENP-N. We report cryo–electron microscopy, biophysical, biochemical, and cell biological studies of the interaction between the CENP-A nucleosome and CENP-N. We show that human CENP-N confers binding specificity through interactions with the L1 loop of CENP-A, stabilized by electrostatic interactions with the nucleosomal DNA. Mutational analyses demonstrate analogous interactions in Xenopus, which are further supported by residue-swapping experiments involving the L1 loop of CENP-A. Our results are consistent with the coevolution of CENP-N and CENP-A, and establish the structural basis for recognition of the CENP-A nucleosome to enable kinetochore assembly and centromeric chromatin organization.
报告人简介：Dr. Jingjun Hong earned his Ph. D in Biology in 2009 from University of Science and Technology of China. He started his Postdoc at National Cancer Institute, National Institutes of Health (NIH) since May 2010, and now he is a Research Fellow. In the last years, Dr. Hong has been working on structural biology of histones and their chaperones and centromere/kinetochore proteins in the chromatin filed. He has published his outstanding works in this field in/on Science and Mol. Cell.
1.Sagar Chittori,† Jingjun Hong,† Hayden Saunders, Hanqiao Feng, Rodolfo Ghirlando, Alexander E. Kelly, Yawen Bai, Sriram Subramaniam. Structural mechanisms of centromeric nucleosome recognition by the inner kinetochore protein CENP-N. Science,doi:10.1126/science.aar2781 († Co-first authorship)
2.Jingjun Hong, Hanqiao Feng, Feng Wang, Anand Ranjan, Jianhong Chen, Jiansheng Jiang, Rodolfo Ghirlando, T. Sam Xiao, Carl Wu, Yawen Bai.The Catalytic Subunit of the SWR1 Remodeler Is a Histone Chaperone for the H2A.Z- H2B Dimer. Molecular Cell 53 (3), (2014), 498-505. Cited by: 28F1000Prime Recommended Article, http://f1000.com/prime/718270679
3.Jingjun Hong, Hanqiao Feng, Zheng Zhou, Rodolfo Ghirlando, Yawen Bai. Identification of Functionally Conserved Regions in the Structure of the Chaperone/CenH3/H4 Complex. Journal of Molecular Biology 425 (3), (2013), 536- 545. Cited by: 7
4.Jingjun Hong, Jiahai Zhang, Zhijun Liu, Su Qin, Jihui Wu, Yunyu Shi. Solution structure of S. cerevisiae PDCD5-like protein and its promoting role in H2O2-induced apoptosis in yeast. Biochemistry 48 (29), (2009), 6824-6834. Cited by: 16