特任教授, 博士生导师。入选国家海外高层次人才青年项目，中科院率先行动引才计划（已获择优支持），安徽省海外创新人才计划等。现任中国生物物理学会冷冻电子显微学分会委员。2010年本科毕业于吉林大学，2015年博士毕业于北京大学。2015年7月-2021年4月先后在美国贝勒医学院、斯坦福大学任博士后、研究科学家。
2021年5月加入中国科学技术大学生命科学与医学部，组建RNA与分子机器结构生物学实验室。课题组主要运用冷冻电镜技术结合生物化学方法解析与重大疾病相关的RNA、RNA-蛋白复合物等生物大分子结构。至今，在国际知名期刊发表论文50余篇，包括Nature、Science、Nature Methods、Molecular Cell、Cell Research、Nature Structural & Molecular Boilogy、Science Advances、Nature Communications、PNAS等杂志。
课题组研究方向：
1. 烈性RNA病毒的RNA元件及与重大疾病相关的RNA/蛋白复合物的结构研究；
2. 与蛋白/核酸质量控制相关的生物大分子机器的结构与机制研究；
3. 开发优化冷冻电镜技术方法和RNA三维结构建模软件。
联系方式：
Email: kmzhang(at)ustc.edu.cn (at 换成@)
招生招聘：
实验室长期招聘具有各类研究背景的优秀博士后，待遇从优。同时热忱欢迎本科生前来攻读博士学位或实习。
发表文章（#:共同第一作者；*:通讯作者, IF: Impact Factor；按时间倒序）
Google Scholar:
https://scholar.google.com/citations?hl=en&user=dRjlvdkAAAAJ&view_op=list_works&sortby=pubdate
代表性论著（仅列举2016-2022年第一（共一）或通讯作者文章；按时间倒序排序；所有共同第一作者均加注上标“#”字样，通讯作者及共同通讯作者均加注上标“*”字样，唯一第一作者且非通讯作者无加注；本人姓名加粗显示）
1. Su S#, Li S#, Deng T#, Gao M, Yin Y, Wu B, Peng C, Liu J, Ma J*, Zhang K*. Cryo-EM Structures of Human m6A Writer Complexes.Cell Res. 2022
2. Li S, Palo MZ, Pintilie G, Zhang X, Su Z, Kappel K, Chiu W*, Zhang K*, Das R*. Topological crossing in the misfolded Tetrahymena ribozyme resolved by cryo-EM. Proc Natl Acad Sci U S A. 2022 Sep 13;119(37):e2209146119. doi: 10.1073/pnas.2209146119. Epub 2022 Sep 6. PMID: 36067294; PMCID: PMC9477386.
3. Guo Q#, Zhao S#, Francisco-Velilla R, Zhang J, Embarc-Buh A, Abellan S, Lv M, Tang P, Gong Q, Shen H, Sun L, Yao X, Min J, Shi Y, Martínez-Salas E*, Zhang K*, Xu C*. Structural basis for Gemin5 decamer-mediated mRNA binding. Nat Commun. 2022 Sep 2;13(1):5166. doi: 10.1038/s41467-022-32883-z. PMID: 36056043; PMCID: PMC9440017.
4. Zhang K#, Li S#, Wang Y, Wang Z, Mulvenna N, Yang H, Zhang P, Chen H, Li Y, Wang H, Gao Y, Wigneshweraraj S, Matthews S, Zhang K*, Liu B*. Bacteriophage protein PEIP is a potent Bacillus subtilis enolase inhibitor. Cell Rep. 2022 Jul 5;40(1):111026. doi: 10.1016/j.celrep.2022.111026. PubMed PMID: 35793626.
5.Zhang K*,#, Horikoshi N#, Li S, Powers S, Hameedi M, Pintilie G, Chae H, Khan Y, Suomivuori C, Dror R, Sakamoto K*, Chiu W*, and Wakatsuki S*. Cryo-EM, Protein Engineering, and Simulation Enable the Development of Peptide Therapeutics against Acute Myeloid Leukemia. ACS Cent. Sci. 2022 Feb 7; doi.org/10.1021/acscentsci.1c01090
6. Li S#, Hsieh KY#, Kuo CI, Su SC, Huang KF, Zhang K*, Chang CI*. Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex. Sci Adv. 2021 Nov 12;7(46):eabj9537. doi: 10.1126/sciadv.abj9537. Epub 2021 Nov 10. PubMed PMID: 34757797.
7. Liu B#*, Li S#, Liu Y#, Chen H, Hu Z, Wang Z, Zhao Y, Zhang L, Ma B, Wang H, Matthews S, Wang Y*, Zhang K*. Bacteriophage Twort protein Gp168 is a β-clamp inhibitor by occupying the DNA sliding channel. Nucleic Acids Res. 2021 Nov 8;49(19):11367-11378. doi: 10.1093/nar/gkab875. PubMed PMID: 34614154; PubMed Central PMCID: PMC8565349.
8. Cogan DP#, Zhang K#, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W*, Khosla C*. Mapping the catalytic conformations of an assembly-line polyketide synthase module. Science. 2021 Nov 5;374(6568):729-734. doi: 10.1126/science.abi8358. Epub 2021 Nov 4. PubMed PMID: 34735239.
9. Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K*, Chang CI*. Complete three-dimensional structures of the Lon protease translocating a protein substrate. Sci Adv. 2021 Oct 15;7(42):eabj7835. doi: 10.1126/sciadv.abj7835. Epub 2021 Oct 15. PubMed PMID: 34652947; PubMed Central PMCID: PMC8519571.
10. Li S, Hsieh KY, Su SC, Pintilie GD, Zhang K*, Chang CI*. Molecular basis for ATPase-powered substrate translocation by the Lon AAA+ protease. J Biol Chem. 2021 Oct;297(4):101239. doi: 10.1016/j.jbc.2021.101239. Epub 2021 Sep 24. PubMed PMID: 34563541; PubMed Central PMCID: PMC8503904.
11.Zhang K#, Zheludev IN#, Hagey RJ#, Haslecker R, Hou YJ, Kretsch R, Pintilie GD, Rangan R, Kladwang W, Li S, Wu MT, Pham EA, Bernardin-Souibgui C, Baric RS, Sheahan TP, D'Souza V, Glenn JS*, Chiu W*, Das R*. Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome. Nat Struct Mol Biol. 2021 Sep;28(9):747-754. doi: 10.1038/s41594-021-00653-y. Epub 2021 Aug 23. PubMed PMID: 34426697. (Cover Story)
12. Su Z#*, Zhang K#, Kappel K#, Li S, Palo MZ, Pintilie GD, Rangan R, Luo B, Wei Y, Das R*, Chiu W*. Cryo-EM structures of full-length Tetrahymena ribozyme at 3.1 Å resolution. Nature. 2021 Aug;596(7873):603-607. doi: 10.1038/s41586-021-03803-w. Epub 2021 Aug 11. PubMed PMID: 34381213; PubMed Central PMCID: PMC8405103.
13. Li H#, Zhang K#, Binzel DW, Shlyakhtenko LS, Lyubchenko YL, Chiu W, Guo P*. RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus. RNA Biol. 2021 Apr 29;:1-11. doi: 10.1080/15476286.2021.1915620. [Epub ahead of print] PubMed PMID: 33845711.
14.Zhang K*, Pintilie GD, Li S, Schmid MF, Chiu W*. Resolving individual atoms of protein complex by cryo-electron microscopy. Cell Res. 2020 Dec;30(12):1136-1139. doi: 10.1038/s41422-020-00432-2. Epub 2020 Nov 2. PubMed PMID: 33139928; PubMed Central PMCID: PMC7605492.
15.Zhang K, Li S, Pintilie G, Chmielewski D, Schmid MF, Simmons G, Jin J, Chiu W*. A 3.4-Å cryo-electron microscopy structure of the human coronavirus spike trimer computationally derived from vitrified NL63 virus particles. QRB Discov. 2020 Nov 17;1:e11. doi: 10.1017/qrd.2020.16. PubMed PMID: 34192263; PubMed Central PMCID: PMC7737156.
16. Kappel K#, Zhang K#, Su Z#, Watkins AM, Kladwang W, Li S, Pintilie G, Topkar VV, Rangan R, Zheludev IN, Yesselman JD, Chiu W*, Das R*. Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures. Nat Methods. 2020 Jul;17(7):699-707. doi: 10.1038/s41592-020-0878-9. Epub 2020 Jul 2. PubMed PMID: 32616928; PubMed Central PMCID: PMC7386730.
17. Partin AC#, Zhang K#, Jeong BC, Herrell E, Li S, Chiu W, Nam Y*. Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA. Mol Cell. 2020 May 7;78(3):411-422.e4. doi: 10.1016/j.molcel.2020.02.016. Epub 2020 Mar 27. PubMed PMID: 32220646; PubMed Central PMCID: PMC7214211.
18.Zhang K#, Wang S#, Li S#, Zhu Y#, Pintilie GD, Mou TC, Schmid MF, Huang Z*, Chiu W*. Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas complex revealed by cryo-EM. Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7176-7182. doi: 10.1073/pnas.1922638117. Epub 2020 Mar 13. PubMed PMID: 32170016; PubMed Central PMCID: PMC7132274.
19. McClelland LJ#, Zhang K#, Mou TC#, Johnston J, Yates-Hansen C, Li S, Thomas CJ, Doukov TI, Triest S, Wohlkonig A, Tall GG, Steyaert J, Chiu W*, Sprang SR*. Structure of the G protein chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1. Nat Commun. 2020 Feb 26;11(1):1077. doi: 10.1038/s41467-020-14943-4. PubMed PMID: 32103024; PubMed Central PMCID: PMC7044438.
20. Guo S#, Vieweger M#, Zhang K#, Yin H, Wang H, Li X, Li S, Hu S, Sparreboom A, Evers BM, Dong Y, Chiu W, Guo P*. Ultra-thermostable RNA nanoparticles for solubilizing and high-yield loading of paclitaxel for breast cancer therapy. Nat Commun. 2020 Feb 20;11(1):972. doi: 10.1038/s41467-020-14780-5. PubMed PMID: 32080195; PubMed Central PMCID: PMC7033104.
21.Zhang K#, Li S#, Kappel K#, Pintilie G, Su Z, Mou TC, Schmid MF, Das R*, Chiu W*. Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution. Nat Commun. 2019 Dec 3;10(1):5511. doi: 10.1038/s41467-019-13494-7. PubMed PMID: 31796736; PubMed Central PMCID: PMC6890682.
22.Zhang K#, Zhang H#, Li S#, Pintilie GD, Mou TC, Gao Y, Zhang Q, van den Bedem H, Schmid MF, Au SWN*, Chiu W*. Cryo-EM structures of Helicobacter pylori vacuolating cytotoxin A oligomeric assemblies at near-atomic resolution. Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):6800-6805. doi: 10.1073/pnas.1821959116. Epub 2019 Mar 20. PubMed PMID: 30894496; PubMed Central PMCID: PMC6452728.
23. Guo M#, Zhang K#, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W*, Huang Z*. Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry. Cell Res. 2019 Apr;29(4):305-312. doi: 10.1038/s41422-019-0151-x. Epub 2019 Feb 27. PubMed PMID: 30814678; PubMed Central PMCID: PMC6461802.
24. Michalska K#, Zhang K#, March ZM#, Hatzos-Skintges C, Pintilie G, Bigelow L, Castellano LM, Miles LJ, Jackrel ME, Chuang E, Jedrzejczak R, Shorter J*, Chiu W*, Joachimiak A*. Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events. Structure. 2019 Mar 5;27(3):449-463.e7. doi: 10.1016/j.str.2018.11.001. Epub 2018 Dec 27. PubMed PMID: 30595457; PubMed Central PMCID: PMC6403000.
25. Jun H#, Shepherd TR#, Zhang K#, Bricker WP, Li S, Chiu W, Bathe M*. Automated Sequence Design of 3D Polyhedral Wireframe DNA Origami with Honeycomb Edges. ACS Nano. 2019 Feb 26;13(2):2083-2093. doi: 10.1021/acsnano.8b08671. Epub 2019 Jan 24. PubMed PMID: 30605605; PubMed Central PMCID: PMC6679942.
26.Zhang K#, Keane SC#, Su Z, Irobalieva RN, Chen M, Van V, Sciandra CA, Marchant J, Heng X, Schmid MF, Case DA*, Ludtke SJ*, Summers MF*, Chiu W*. Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach. Structure. 2018 Mar 6;26(3):490-498.e3. doi: 10.1016/j.str.2018.01.001. Epub 2018 Feb 2. PubMed PMID: 29398526; PubMed Central PMCID: PMC5842133.
27. Li H#, Zhang K#, Pi F, Guo S, Shlyakhtenko L, Chiu W, Shu D, Guo P*. Controllable Self-Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting. Adv Mater. 2016 Sep;28(34):7501-7. doi: 10.1002/adma.201601976. Epub 2016 Jun 20. PubMed PMID: 27322097; PubMed Central PMCID: PMC5059845.
28. Veneziano R#, Ratanalert S#, Zhang K#, Zhang F, Yan H, Chiu W, Bathe M*. Designer nanoscale DNA assemblies programmed from the top down. Science. 2016 Jun 24;352(6293):1534. doi: 10.1126/science.aaf4388. Epub 2016 May 26. PubMed PMID: 27229143; PubMed Central PMCID: PMC5111087.
