龙 冬

 


教授,博士生导师。2005年毕业于武汉大学获得学士学位;2010年于新加坡国立大学获得博士学位;20102015先后于美国国家强磁场实验室和加拿大多伦多大学从事博士后研究,期间获得加拿大健康研究院基金资助(CIHR Fellowship)2015年加入中国科学技术大学生命科学学院及合肥微尺度物质科学国家研究中心。2018年获得国家自然科学基金委“优秀青年科学基金”资助。多年来一直从事核磁共振波谱学和生物物理化学领域的研究,PNASJ Am Chem SocAngew Chem Int EdJ Phys Chem Lett国际学术刊物发表了系列文章。近期工作获得Faculty Opinions(原F1000Prime推荐,并受到学术界和制药公司同行的关注。 

 

主要研究兴趣

我们的科研工作处于生命科学、物质科学计算科学的交叉领域。利用前沿的多维核磁共振波谱学技术、计算机模拟和多种生物物理、生物化学手段定量研究核酸、蛋白质等大分子的空间三维结构,并且探测这些结构随着时间(皮秒至秒的时间尺度)的动态变化过程。我们工作中所获取的生物大分子结构和构象动态信息对于理解生物分子行使其生理功能的机制机理以及针对生物大分子的药物研发都意义重大。

 

近期主要论文

1. D. Liu, Y. Mao, X. Gu, Y. Zhou, D. Long* (2021) Unveiling the invisible druggable conformations of GDP-bound inactive Ras. Proc. Natl. Acad. Sci. U.S.A. 118(11):e2024725118

2. X. Chen, H. Gao, D. Long* (2021) Millisecond Allosteric Dynamics of Activated Ras Reproduced with a Slowly Hydrolyzable GTP Analogue. ChemBioChem 22:1079-1083

3. D. Liu, X. Chen, D. Long* (2020) NMR-Derived Conformational Ensemble of State 1 of Activated Ras Reveals Insights into a Druggable Pocket. J. Phys. Chem. Lett. 11:3642-3646

4. X. Chen, H. Yao, H. Wang, Y. Mao, D. Liu, D. Long* (2019) Extending the lifetime of native GTP-bound Ras for site-resolved NMR measurements: Quantifying the allosteric dynamics. Angew. Chem. Int. Ed. 58:2730-2733

5. P. Cheng, D. Liu, P. X. Chee, D. Yang, D. Long* (2019) Atomistic Insights into the Functional Instability of the Second Helix of Fatty Acid Binding Protein. Biophys. J. 117:239-246

6. S. Li, R. Hu, H. Yao, D. Long, F. Luo, X. Zhou, X. Zhang, M. Liu, J. Zhu, Y. Yang (2018) Characterization of the interaction interface and conformational dynamics of human TGIF1 homeodomain upon the binding of consensus DNA. BBA - Proteins Proteom. 1866:1021-1028

7. H. Wang, S. Wang, C. Li, H. Li, Y. Mao, W. Liu, C. Xu*, D. Long* (2017) Probing transient release of membrane-sequestered tyrosine-based signaling motif by solution NMR spectroscopy. J. Phys. Chem. Lett. 8:3765-3769

8. Y. Mao, H. Yao, H. Wang, P. Cheng, D. Long* (2016) Microsecond timescale dynamics of GDP-bound Ras underlies the formation of novel inhibitor-binding pockets. Angew. Chem. Int. Ed. 55:15629-15632

9D. Long,* F. Delaglio, A. Sekhar, L. E. Kay* (2015) Probing invisible, excited protein states by non-uniformly sampled pseudo-4D CEST spectroscopy. Angew. Chem. Int. Ed. 54:10507-10511

10. D. Long, A. Sekhar, L. E. Kay (2014) Triple resonance-based 13Cα and 13Cβ CEST experiments for studies of ms timescale dynamics in proteins. J. Biomol. NMR 60:203-208

11. D. Long, G. Bouvignies, L. E. Kay (2014) Measuring hydrogen exchange rates in invisible protein excited states. Proc. Natl. Acad. Sci. U.S.A. 111:8820-8825

12. D. Long, C. B. Marshall, G. Bouvignies, M. T. Mazhab-Jafari, M. J. Smith, M. Ikura, L. E. Kay. (2013) A comparative CEST NMR study of slow conformational dynamics of small GTPases complexed with GTP and GTP analogues. Angew. Chem. Int. Ed. 52:10771-10774

13. H. Sun, D. Long, R. Brüschweiler, V. Tugarinov. (2013) Carbon relaxation in 13Cα-Hα and 13Cα-Dα spin pairs as a probe of backbone dynamics in proteins. J. Phys. Chem. B 117:1308-1320

14. D. Long, R. Brüschweiler. (2013) Directional selection precedes conformational selection in ubiquitin-UIM binding. Angew. Chem. Int. Ed. 52: 3709-3711

15. D. Long, R. Brüschweiler. (2012) Structural and entropic allosteric signal transduction strength via correlated motions. J. Phys. Chem. Lett. 3:1722-1726

16. D. Long, R. Brüschweiler. (2011) Atomistic kinetic model for population shift and allostery in biomolecules. J. Am. Chem. Soc. 133:18999-19005

17. D. Long, D.W. Li, K. Walter, C. Griesinger, R. Brüschweiler. (2011) Toward a predictive understanding of slow methyl group dynamics in proteins. Biophys. J. 101:910-915

18. D. Long, R. Brüschweiler. (2011) In silico elucidation of the recognition dynamics of ubiquitin. PLoS Comput. Biol. 7: e1002035

 

联系方式:

Email: dlong[at]ustc.edu.cn

 

 


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